Influence of microrays on the fibrinolytic activities of streptokinase and streptodornase.

The results of our study may briefly be summarized as follows: 1) The irradiation with microrays (20∼30 watts) similar as 2,000 R and 5,000 R Gamma radiation did not substantially affect the activity of fibrinolysin (SK+SD). 2) By the irradiation method so far mentioned it has been demonstrated that the fibrinolytic activity of anticoagulant of the SK+SD preparation is preserved in all the clotting systems which we used. 3) Our findings indicate that it is possible to irradiate patients for therapeutical purpose with Radarmed (electromagneticrays) provided that there is produced some enhancing influence of the same blood clotting factors or systems. Together with earlier works in this field it appears that this method of the microirradiation could provide us with an important evidence on which we can base our further in vitro and in vivo radiohematologic studies; investigations with various preparations, types of radiation that are still underway9∼16. ∗PMID: 4230847 [PubMed indexed for MEDLINE] Copyright c ©OKAYAMA UNIVERSITY MEDICAL SCHOOL Acta Med. Okayama 21, 161-166 (1967) INFLUENCE OF MICRORAYS ON THE FIBRINOLYTIC ACTIVITIES OF STREPTOKINASE AND STREPTODORNASE Endre SZIRMAI* and Srdjan HAJDUKOVIC** Department of Nuclear Hematology and Radiation Biology, Institute of Nuclear Energy, Stuttgart, F. R., Germany and University of **Department of Radiation Biology, Institut of Nuclear Sciences, "Boris Kidrich", Vinca, Belgrad, Yugoslavia Received for publication, July 21, 1967 In the research field of radiation biology we have already reported in several papers about the disturbances of blood clotting or the arrest of blood clotting factors and systems under the influences of various types and dosages of radiation ,12-16. Such disturbances of the blood clotting system also seem to occur in the case of microray irradiation which is now common for the treatment of various diseases by using Radar Med. of Elektronik GmbH. Berlin and other companies. The microray irradiation proved to be very effective to cure the hematomas and other pathological conditions, but small purpuras are sometimes met with in the patients treated with the microray. Thus, there is great possibility that the microray irradiation may also induce the disturbances of blood clotting system resulting in the unfavorable side-effect, the appearance of purpura. So we attempted to check the possible effect of the microray on the blood clotting system. And we have observed individual partial problems of fibrinolysis in vitro but failed to obtain any evidence of the suspected effect of irradiation on various fibrinolysin preparations. Therefore, we first irradiated Varidase (American Cyanamid Company), Streptokinase-Streptodornase (Lederle, United States and Munich) in the doses of 2,000 Rand 5,000 R. In this paper we present briefly the results of our studies with Varidase after the microray-irradiation. MATERIAL AND METHODS Two different batches of Varidase, each vial containing 20,000 units of streptokinase and 5,000 units of streptodornase were used. The following tests were applied as in a previous paper 5, but thrombelasto* Parmanent address: 11 Adolf-Kroner-Str., Stuttgart·O, F. R, Germany 161 1 Szirmai and Hajdukovic: Influence of microrays on the fibrinolytic activities of streptok Produced by The Berkeley Electronic Press, 1967 162 E. SZIRMAI and S. HAJDUKOVIC graphy was also employed in order to study the effect of Varidase on blood clotting and fibrinolysis before and after irradiation: (a) Clotting time of recalcinated plasma ; (b) thrombin clotting time ; (c) fibrinolytic activity on non-heated fibrin plates ; (d) auto-coagulogram ; (e) thrombelastographyt7. Vials containing streptokinase were irradiated by the Radarmed (of Dr. Szirmai). The specifications of the equipment used are as follows: Microrays apparatus of Deutschen (German) Electronic CmbH., Berlin, TS 5302. The apparatus produces 12.4 cm long rays at the frequency of 2400 MHz. The amplitudes will be produced by the so-called "Ganzmetall-Mehrschlitz-Magnetoron" and due to the Radiator by a total, isolated coaxial label. The action intensity is between 1r-200 watts. We usually use 20r-30 watts. Studies were carried out to determine different blood clotting factors in patients before and after irradiation and also the activities of non-irradiated and irradiated streptokinase -streptodornase, 4, 48 and 96 hr respectively after irradiation. The streptokinase-streptodornase was dissolved in 20 ml of isotonic saline solution as in our pervious examinations, which was added to each vial. The vials were refrigerated when not in use. In this paper only the results of our in vitro examinations are described. To determine the clotting time of recalcinated plasma 0.1 ml of streptokinase was added to 0.2 ml of normal plasma and the mixture incubated for 30 min; 0.3 ml of CaCl2 (M/40) was then added and the clotting time determined. Table 1 (SZIRMAI and HAJDUKOVIC) 1,1 Subject I ! Subject II Material I Material II I NonI Irradiated I I NonI Control irradiated 4 h I Control irradiated Clotting time of recalI 74 211/202 20-30 Watt II 182 191/190 cinated plasma (seconds) 211/209 Thrombin clotting time 20 more than more than 23 more than I (seconds) 10 min. 10 min. 10 min. Fibrinolytic activity 134/133 113/116 222/219 I (field in mm2) I i Auto coagulogram (%) incubation time 26 29/31 23/22 22 36/41 4 10 101 57/63 64/64 103 79/81 20 102 33/35 31/30 104 54/52 60 I 42 19/22 11/12 33 20/21 2 Acta Medica Okayama, Vol. 21 [1967], Iss. 4, Art. 2 http://escholarship.lib.okayama-u.ac.jp/amo/vol21/iss4/2 Influence of Microrays on the Fibrinolytic Activities 163 38/40 79/81 For the thrombin clotting time 0.2 ml of plasma was incubated with 0.1 ml of streptokinase and then added with 0.3 ml of thrombin (Antithrombin Reagent Roche; 16 units dissolved in 3 ml of distilled water). The fibrin plates were prepared with ox fibrinogen (Warner Chilcott) and thrombin. The substrate plasma used for the autocoagulogram consisted of one ml of plasma from the subject, mixed with 0.1 ml of streptokinase-streptodornase, incubated for 30 min and used as previously described• For the controls in all the tests, an identical amount of the isotonic saline solution was added instead of streptokinase-streptodornase. As far as the autocoagulogram method is concerned, our work was facilitated by determining the clotting time after 4, 10, 20 or 60 min, respectively of incubation of the hemolysates and the results are given as coagulation activity in per cent (Fig. 1).